BIM A Product: Study of the Operation BIM Product on the Modulation of the Immune System
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BIMA products are immune modulators for autoimmune issues and also arthritis. Studies show reduced production of IL-2 and IL-17 and significantly reduced the production of TNF-a “Arthrinok reduces excessive TNF-α, IL-6, and IL17 which cause auto-immune diseases including arthritis.” Prof. Dr. Pichaet Wiriyachitra Ph.D., F.R.A.C.I
60 Capsules Per Box
Sesame Extract 200 mg (33.33%)
Centella Asiatic Powder 104 mg. (17.34%)
Protein extract Soy 100 mg. (16.67%)
Guava juice is 50 mg. (8.33%)
Mangosteen juice powder 50 mg. (8.33%)
Directions: Take 2 capsules, 2-3 times a day, 30 minutes to 1 hour before meal.
During the last decades, development of specific agents affecting T helper cell subpopulations, i.e., Th1, Th2 and Th17, differentiation has drawn special attention. Many natural products were reported as a good agent for modulating the immune response by regulating the differentiation of T helper cell subpopulations. These products are potential to be the immunomodulators for treatment of various diseases including infectious diseases, cancers, autoimmune diseases and also arthritis.
In this study, we aim to study the immunomodulatory effects of the Operation BIM product. We investigate the possible effect of an Operation BIM product, Arthrinox, on the controlling of T helper cell subpopulation differentiation.
The objective of this study is to investigate the possible effects of the BIM A Product on the regulation of T helper cell subpopulations. The levels of various cytokines of blood collected from healthy donors before and after taking Operation BIM product (Arthrinox capsule) for 15 days were compared.
1. Study subjects
• 12 healthy volunteers: 6 males and 6 females
• Age: 20-55 years old
• The recruited volunteers were separated into 2 groups:
Group 1: taking placebo; 6 subjects
Group 2: taking Arthrinox capsule; 6 subjects
2. Blood collections
Blood (5 ml. using heparin as anticoagulant) were collected from each subject at day 0. According to their groups, subjects took Arthrinox capsule or placebo (4 capsules/day) every day for 15 days. Afterward, blood was collected (5 ml. using heparin as anticoagulant) for the second time at day 16.
3. Study of the effect of the Arthrinox capsule on the regulation of T helper cell subpopulations
Peripheral blood mononuclear cells (PBMCs) were isolated from the collected blood by using Ficoll-Hypaque gradient centrifugation. PBMCs were in vitro stimulated with or without anti-CD3 monoclonal antibodies (clones OKT3) and cultured for 24 hours at 37oC in a CO2 incubator. The cell culture supernatant was collected and centrifuged 20,000 rpm, 2 min. The cell-free supernatants were separated and stored at -70°C for determination of cytokines.
The stimulation indexes of various cytokines in subjects taking the Arthrinox capsules and placebo were calculated and compared. By normalization using subjects taking placebo, % enhancement and reduction of subjects taking Arthrinox capsule were shown in Figure below. The results indicate that Arthrinox capsule reduced the production of IL-2 and IL-17 and significantly reduced the production of TNF-a.
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